Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs

Complemented palindrome small RNAs first discovered from SARS coronavirus

1. Laboratory of Medical Molecular Virology, School of Medicine, Nankai University, Tianjin, Tianjin 6 300071, P.R.China. 7 2. State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Veterinary Parasitology of 8 Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, 9 Lanzhou, Gansu 730046, P.R.China. 10 3. Co-innovation Center for P...

Small Interfering RNA Effectively Inhibits the Expression of SARS Coronavirus Membrane Gene at Two Novel

Small interfering RNA (siRNA) is a class of duplex RNA molecules of 21-25 nt nucleotides in length functioning post-transcriptionally to downregulate targeted gene expression. The membrane (M) protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is highly abundant during viral infections and is a critical element for viral assembly. Nucleotide substitution in the viral...

stably silencing of cd81 expression by small interfering rnas targeting 3'-ntr inhibits hcv infection

Delivery and inhibition of reporter genes by small interfering RNAs in a mouse skin model.

RNA interference offers the potential of a novel therapeutic approach for treating skin disorders. To this end, we investigated delivery of nucleic acids, including a plasmid expressing the reporter gene luciferase, to mouse skin by intradermal injection into footpads using in vivo bioluminescence imaging over multiple time points. In order to evaluate the ability of RNA interference to inhibit...

Effect of small interfering RNAs on in vitro replication and gene expression of feline coronavirus.

OBJECTIVE To evaluate the ability of small interfering RNAs (siRNAs) to inhibit in vitro viral replication and gene expression of feline coronavirus (FCoV). SAMPLE Cell cultures of Crandell-Rees feline kidney cells. PROCEDURES 5 synthetic siRNAs that each targeted a different region of the FCoV genome were tested individually and in various combinations for their antiviral effects against 2...